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Invitrogen™ Dynabeads™ Regulatory CD4+/CD25+ T Cell Kit

Product Code. 10154833
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2 x 5 mL
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This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price
Product Code. 10154833 Supplier Invitrogen™ Supplier No. 11363D

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700.04 GBP valid until 2026-03-06
BEST PRICE on Promo! Use promo code "27968" to get your promotional price.
This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”27968” to get your promotional price

Description

Includes

2mL Antibody Mix Human CD4, 10mL Depletion MyOne; Dynabeads, 5mL Dynabeads CD25 and 2mL DETACHaBEAD™. These reagents are sufficient to process approx. 1 x 109 cells

Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3.

Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3. The CD4+ CD25- cell fraction can be used as effector cells in downstream inhibitory assays. You can expect >95% purity (CD4+ CD25+ expression) and >80% Foxp3 expression.

Isolate bead- and antibody-free human CD4+ CD25+ Foxp3+ Treg cells
• Treg cells can be expanded with Dynabeads™ CD3/CD28 Treg Expander and remain
Foxp3+.
• Treg cells can be used in any downstream applications (e.g., suppression assays)

Methodology
The isolation procedure includes the following steps:
• Negative isolation of untouched human CD4+ T-cells from PBMC.
• Positive isolation of CD25+ T-cells from the untouched CD4+ T-cell subset.
• Removal of beads from the CD4+CD25+ Treg cells with DETACHaBEAD™

Starting sample
PBMC

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Cell Type T Cells (Regulatory)
Content And Storage Each Kit Contains:
• 2 mL Antibody Mix Human CD4
• 10 mL Depletion MyOne™ Dynabeads™
• 5 mL Dynabeads™ CD25
• 2 mL DETACHaBEAD™.

These reagents are sufficient to process ∼1 x 109 cells. Store components at 2°C to 8°C.
Product Type Cell Isolation Kit
Isolation Technology Depletion, then positive isolation
No. of Cells Processes ∼1 x 109 cells total
Output Viability >95%
Product Line DETACHaBEAD, DYNAL, Dynabeads, FlowComp
Purity or Quality Grade Research Grade
Quantity 2 x 5 mL
Sample Type PBMC
Shipping Condition Room Temperature
Starting Material Cell No. 1 x 108 PBMCs per isolation
Target Species Human
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For Research Use Only. Not for use in diagnostic procedures.

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