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Invitrogen™ Dynabeads™ Regulatory CD4+/CD25+ T Cell Kit

Product Code. 10154833
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Quantity:
2 x 5 mL
Unit Size:
Each
This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 

Product Code. 10154833

Brand: Invitrogen™ 11363D

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This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 

Description

Includes

2mL Antibody Mix Human CD4, 10mL Depletion MyOne; Dynabeads, 5mL Dynabeads CD25 and 2mL DETACHaBEAD™. These reagents are sufficient to process approx. 1 x 109 cells

Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3.

Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3. The CD4+ CD25- cell fraction can be used as effector cells in downstream inhibitory assays. You can expect >95% purity (CD4+ CD25+ expression) and >80% Foxp3 expression.

Isolate bead- and antibody-free human CD4+ CD25+ Foxp3+ Treg cells
• Treg cells can be expanded with Dynabeads™ CD3/CD28 Treg Expander and remain
Foxp3+.
• Treg cells can be used in any downstream applications (e.g., suppression assays)

Methodology
The isolation procedure includes the following steps:
• Negative isolation of untouched human CD4+ T-cells from PBMC.
• Positive isolation of CD25+ T-cells from the untouched CD4+ T-cell subset.
• Removal of beads from the CD4+CD25+ Treg cells with DETACHaBEAD™

Starting sample
PBMC

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Cell Type T Cells (Regulatory)
Content And Storage Each Kit Contains:
• 2 mL Antibody Mix Human CD4
• 10 mL Depletion MyOne™ Dynabeads™
• 5 mL Dynabeads™ CD25
• 2 mL DETACHaBEAD™.

These reagents are sufficient to process ∼1 x 109 cells. Store components at 2°C to 8°C.
Product Type Cell Isolation Kit
Isolation Technology Depletion, then positive isolation
No. of Cells Processes ∼1 x 109 cells total
Output Viability >95%
Product Line DETACHaBEAD, DYNAL, Dynabeads, FlowComp
Purity or Quality Grade Research Grade
Quantity 2 x 5 mL
Sample Type PBMC
Shipping Condition Room Temperature
Starting Material Cell No. 1 x 108 PBMCs per isolation
Target Species Human
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For Research Use Only. Not for use in diagnostic procedures.

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