Cloned AMV First-Strand cDNA Synthesis Kit

Combines the high performance of Invitrogen Cloned AMV RT with the convenience of optimized, prequalified reagents

Overview
Brand: Invitrogen

Manufacturer Part Number: 12328032

25 RXN CLONED AMV FIRST STRAND SYNTHESIS KIT 25reactions Store at -68C to -85C

UNSPSC: 12352204

Code: 50

Additional Details:
Additional Details: Weight: 0.00454kg



Product Code. 10175092

Quantity Price
1 £ 144.0 / 25 reactions
Estimated Shipment date
from Supplier 12-12-2016
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For Research Use Only. All usage must comply with product instructions.
 

Description and Specification

Specification

For Use With (Application) First-Strand cDNA Synthesis, RT-PCR
Format Frozen
Includes 25μL cloned AMV RT (15U/μL), 500μL 5X cDNA synthesis buffer, 250μL 0.1M DTT, 100μL 10mM dNTP mix, 25μL RnaseOUT (40U/μL), 25μL oligo(dT)20 (50μM), 50μL random hexamers (50ng/μL), 1.25mL DEPC-treated water, product profile sheet
Packaging Type Tube
Quantity 25 reactions (375 units)
Storage Requirements Store at -70deg.C
Temperature (Metric) Operating 45 to 55deg.C
Test Sensitivity Low

The subunits of the avian myeloblastosis virus gene were co-expressed and purified to produce this recombinant AMV RT. Unlike the commonly available native enzyme, Invitrogen Cloned AMV is a high-purity preparation that results in greater specific activity, leading to improved yields and superior sensitivity. To achieve high-quality routine RT-PCR, combine with Platinum Taq DNA Polymerase for high-specificity PCR or with Platinum Taq DNA Polymerase High Fidelity for subsequent PCR cloning.

  • Uses high-purity Cloned AMV for higher yields
  • Includes 5X first-strand buffer with proprietary agents for more robust performance
  • Replaces the cDNA Cycle Kit and AMV Reverse Transcriptase System
  • Detects transcripts in 1.0pg of RNA
  • Detects various mRNA targets ranging in size from 1.6kb to 9.4kb
  • Concentration: 15U/μL

  • Optimal reaction temperature: 45-55°C
  • Final product size: 9.4kb or less

PCR & Real-Time PCR, Reverse Transcription