Store at -20° or -80°C. Guaranteed stable for 6 months when properly stored.
Proteins catalyze in vitro recombination of PCR products or DNA segments from clones (containing attB sites) and Donor vector (containing attP sites) to generate Entry clones
BP Clonase II enzyme format is single mix of both enzyme and buffer to ensure enzyme stability and enable convenient 10μL reaction set up with fewer pipetting steps, which both maximize recombination efficiency and minimize pippetting
Original format of BP Clonase enzyme is stand alone enzyme blend and reaction buffer provided in separate tubes