CIAP (Calf Intestinal Alkaline Phosphatase)

Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA

Brand: Invitrogen

Manufacturer Part Number: 18009027

CALF INT. ALK. PHOSPH.(1 units/ ┬Ál)

UNSPSC: 12352204

Code: 50

Additional Details:
Additional Details: Weight: 0.02000kg

Product Code. 10593263

Quantity Price
1 £69.75 / Each
EU Stock 2
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For Research Use Only. All usage must comply with product instructions.

Description and Specification


Concentration 1U/μL
Quantity 1000 units
Storage Requirements Store at -20°C

  • Concentration: 20 units/μL
  • Source: Purified from calf intestinal mucosa
  • Performance and Quality Testing: Endodeoxyribonuclease, 3' exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay
  • Unit Definition: One unit hydrolyzes 1μmol of 4-nitrophenyl phosphate in 1 min. at 37°C
  • Unit Reaction Conditions: 1M diethanolamine buffer, 10mM 4-nitrophenyl phosphate, 0.25mM MgCl2 (pH 9.8) in 900μL for 10 min. at 37°C

Dephosphorylation of 5'-phosphorylated termini of vector DNA to prevent self-ligation; Dephosphorylation of 5' termini of nucleic acids prior to forward reaction with kinase; Cloning; Restriction Enzyme Cloning