CIAP (Calf Intestinal Alkaline Phosphatase)

Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA

Brand: Invitrogen

Manufacturer Part Number: 18009019


UNSPSC: 12352204

Code: 50

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 10747864

Quantity Price
1 £70.75 / 1ku
Estimated Shipment date
from Supplier 25-10-2016
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For Research Use Only. All usage must comply with product instructions.

Description and Specification


Concentration 20U/μL
Includes 10X Dephosphorylation Buffer [500mM Tris-HCl (pH 8.5), 1mM EDTA] and Dilution Buffer [50% glycerol, 25mM Tris-HCl (pH 7.6), 1mM MgCl2 , and 0.1mM ZnCl2]
Quantity 1000 units
Storage Requirements Store at -20°C

  • Concentration: 20 units/μL
  • Source: Purified from calf intestinal mucosa
  • Performance and Quality Testing: Endodeoxyribonuclease, 3' exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay
  • Unit Definition: One unit hydrolyzes 1μmol of 4-nitrophenyl phosphate in 1 min. at 37°C
  • Unit Reaction Conditions: 1M diethanolamine buffer, 10mM 4-nitrophenyl phosphate, 0.25mM MgCl2 (pH 9.8) in 900μL for 10 min. at 37°C

Dephosphorylation of 5'-phosphorylated termini of vector DNA to prevent self-ligation; Dephosphorylation of 5' termini of nucleic acids prior to forward reaction with kinase; Cloning; Restriction Enzyme Cloning