Invitrogen™ ChargeSwitch™ PCR Clean-Up Kit

Product Code.	No. of Reactions	unitSize
10685273	100 Preps	Each
10221853	960 Preps	Each

Product Code. 10685273 Supplier Invitrogen™ Supplier No. CS12000

Description

Includes

ChargeSwitch Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
ChargeSwitch Purification Buffer (N5)
ChargeSwitch Wash Buffer (W12)
ChargeSwitch Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

Requires

Magnetic accessory

ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts.

ChargeSwitch PCR Clean-Up Kit is the fastest and most effective method of removing primers, unincorporated dNTPs, thermostable polymerases and salts (Figure 1). This kit is scalable for use on liquid-handling robots. This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.

• Sample size: 25–50 μL
• High recovery of DNA fragments
• Rapid, one tube, 5 minute protocol

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage:

  The ChargeSwitch™ PCR Clean-Up Kit is shipped at room temperature. Upon receipt, store all components at room temperature. All components are guaranteed stable for 6 months when stored properly.
  
  Components:
  • ChargeSwitch™ Magnetic Beads (25 mg/mL in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
  • ChargeSwitch™ Purification Buffer (N5)
  • ChargeSwitch™ Wash Buffer (W12)
  • ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)

• Format: Kit
• High-throughput Compatibility: High-throughput Compatible
• Product Line: ChargeSwitch
• Product Type: PCR Clean-Up Kit
• Quantity: 100 Preps
• Run Time: 5 min
• Shipping Condition: Room Temperature
• Target: DNA from Enzymatic Reactions (e.g. PCR)
• For Use With (Application): Next-Generation Sequencing (NGS), Cloning, Standard PCR
• Isolation Technology: ChargeSwitch™ Chemistry
• No. of Reactions: 100 Preps
• Sample Type: PCR Products

Frequently Asked Questions (FAQs)

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.

For Research Use Only. Not for use in diagnostic procedures.