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Invitrogen™ Biotin-14-dCTP
Description
Includes
Biotin-14-dCTP is supplied at 0.4mM in 100mM Tris-HCl (pH 7.5), 0.1mM EDTA.
About Biotin-14-dCTP
Biotin-14-dCTP is a patented dCTP analog with biotin attached at the N4-position of the pyrimidine base by a 14-atom linker. This nucleotide can be efficiently incorporated into DNA by nick translation in the presence of dATP, dGTP, and dTTP, labeling DNA with terminal deoxynucleotide transferase, or random primer synthesis. The amount of material provided is sufficient for labeling up to 50μg of DNA.
Performance and quality testing
Purity is evaluated by reverse-phase HPLC.
- Convenience and safety: probe preparation generates no radioactive waste
- Stability: probes remain active longer than isotopically labeled probes
- Versatility: biotinylated DNA can be used for immobilization and capture procedures via streptavidin binding
DNA and RNA Purification and Analysis, DNA Labeling, Nucleic Acid Labeling and Oligo Synthesis
Order Info
Shipping Condition: Dry Ice
Specifications
Specifications
| Product Type | Labeled Nucleotide |
| Concentration | 0.4 mM |
| Content And Storage | Biotin-14-dCTP is supplied at 0.4 mM in 100 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Store at -20°C. |
| Label or Dye | Biotin & Other Hapten |
| Labeling Method | Direct Labeling |
| Shipping Condition | Dry Ice |
| Quantity | 50 nmol |
| Form | Liquid |
For Research Use Only. Not for use in diagnostic procedures.
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