Invitrogen™ BioPrime™ Total Genomic Labeling Module

Description

Supports a complete genomic DNA labeling system for use in array comparative genomic hybridization applications (aCGH) that allow for better call rates with precious samples

The BioPrime Total Genomic Labeling Module is part of the system that offers users the following advantages:

• Higher signal to noise with Alexa Fluor™ 3 and 5 dyes
• Less channel bias due to novel reaction formulation
• Widest range of input material (50ng - 3μg)
• Simplified workflow with master mix formulation

This complete, all-inclusive kit is optimized to work across the widest range of sample input material with no need for pre-amplification. Highly concentrated exo-Klenow fragment of DNA polymerase I in a component-limited reaction allows for consistent, robust DNA yields of approx. 8μg with as little as 50ng of genomic DNA input. An optimized dye-labeled nucleotide mix with new dNTP linkers, novel application-specific Alexa Fluor 3 and 5 dyes, and improved buffer chemistry reduce labeling variation and increase signal to noise on arrays. Excitation and emission spectra of Alexa Fluor 3 and 5 dyes are suited for conventional two color scanners with no need to change settings. New master mix formulation streamlines reaction set-ups, increasing consistency. Complete kits are batch tested to assure quality in performance from lot to lot.
Contents and Storage:
The BioPrime™ Total Genomic Labeling Module contains Alexa Fluor 3 and Alexa Fluor 5 2x reaction mix, Exo-Klenow fragment (40 U/μL), 5mM EDTA, TE Buffer, and control DNA (Salmon Sperm). Available in a direct labeling format.

Array CGH, ChIP-on-Chip, Chromatin Biology, DNA and RNA Purification and Analysis, DNA Labeling, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Nucleic Acid Labeling and Oligo Synthesis, RNAi, Epigenetics and Non-Coding RNA Research

Specifications

• Detection Method: Fluorescence
• Label or Dye: Alexa Fluor 3, Alexa Fluor 5
• Final Product Type: Probes (Labeled DNA)
• Labeling Target: Genomic DNA
• Includes Label or Dye: Yes
• Labeling Method: Direct Labeling
• Product Line: BioPrime™
• Product Type: Total Genomic Labeling Module
• Quantity: 30 Reactions

For Research Use Only. Not for use in diagnostic procedures.