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Invitrogen™ BioPrime™ Total Genomic Labeling Module

Product Code. 10008322
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30 Reactions
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10008322 30 Reactions Each
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For Research Use Only. All usage must comply with product instructions.
 
Product Code. 10008322 Supplier Invitrogen™ Supplier No. 18097012

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This item is not returnable. View return policy
For Research Use Only. All usage must comply with product instructions.
 

Description

Supports a complete genomic DNA labeling system for use in array comparative genomic hybridization applications (aCGH) that allow for better call rates with precious samples

The BioPrime Total Genomic Labeling Module is part of the system that offers users the following advantages:

  • Higher signal to noise with Alexa Fluor™ 3 and 5 dyes
  • Less channel bias due to novel reaction formulation
  • Widest range of input material (50ng - 3μg)
  • Simplified workflow with master mix formulation
This complete, all-inclusive kit is optimized to work across the widest range of sample input material with no need for pre-amplification. Highly concentrated exo-Klenow fragment of DNA polymerase I in a component-limited reaction allows for consistent, robust DNA yields of approx. 8μg with as little as 50ng of genomic DNA input. An optimized dye-labeled nucleotide mix with new dNTP linkers, novel application-specific Alexa Fluor 3 and 5 dyes, and improved buffer chemistry reduce labeling variation and increase signal to noise on arrays. Excitation and emission spectra of Alexa Fluor 3 and 5 dyes are suited for conventional two color scanners with no need to change settings. New master mix formulation streamlines reaction set-ups, increasing consistency. Complete kits are batch tested to assure quality in performance from lot to lot.
Contents and Storage:
The BioPrime™ Total Genomic Labeling Module contains Alexa Fluor 3 and Alexa Fluor 5 2x reaction mix, Exo-Klenow fragment (40 U/μL), 5mM EDTA, TE Buffer, and control DNA (Salmon Sperm). Available in a direct labeling format.

Array CGH, ChIP-on-Chip, Chromatin Biology, DNA and RNA Purification and Analysis, DNA Labeling, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Nucleic Acid Labeling and Oligo Synthesis, RNAi, Epigenetics and Non-Coding RNA Research

TRUSTED_SUSTAINABILITY

Specifications

Detection Method Fluorescence
Label or Dye Alexa Fluor 3, Alexa Fluor 5
Final Product Type Probes (Labeled DNA)
Labeling Target Genomic DNA
Includes Label or Dye Yes
Labeling Method Direct Labeling
Product Line BioPrime
Product Type Total Genomic Labeling Module
Quantity 30 Reactions

For Research Use Only. Not for use in diagnostic procedures.

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