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Invitrogen™ BaculoDirect™ N-Term Transfection Kit

Product Code. 10042792
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5 transfections
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Product Code. 10042792

Brand: Invitrogen™ 12562062

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Description

Includes

Five 0.3μg vials of BaculoDirect Linear DNA, 125μL of Cellfectin transfection reagent, and ganciclovir.

Powerful and versatile eukaryotic system for high-level protein expression in insect cells

  • Engineered to include attR sites for quick and efficient recombination with Gateway entry clone
  • Gene of interest is recombined from entry clone into BaculoDirect Linear DNA using a simple, one-hour LR reaction
  • Resulting reaction mix contains recombinant baculovirus carrying gene of interest and is used directly to transfect insect cells
  • Need for transforming bacteria and isolating large bacmid or co-transfection of a transfer vector and linear baculovirus DNA into insect cells is eliminated for reduced hands-on time
  • Purified baculovirus can be isolated in less than one week
  • N-terminal V5-His tag for detection with Anti-V5 Antibody and purification with nickel-chelating resin (BaculoDirect N-Term Linear DNA)
  • TEV protease cleavage site for removal of V5-His tag following purification (BaculoDirectN-Term Linear DNA)
  • C-terminal V5-His tag for detection with Invitrogens Anti-V5 Antibody and purification with nickel-chelating resin (BaculoDirect C-Term Linear DNA)
  • Expression can be detected as early as first baculovirus infection

  • Gateway Linear DNA: Designed for rapid cloning with a Gateway entry clone and subsequent expression in Sf9 or Sf21 insect cells
  • Strong polyhedrin promoter for high-level expression
  • R sites for efficient recombination with any attL-flanked Gateway entry vector
  • TK gene for negative selection using ganciclovir
  • N-terminal V5-His tag for detection with Invitrogens Anti-V5 Antibody and purification with nickel-chelating resin (BaculoDirect N-Term Linear DNA)
  • TEV protease cleavage site for removal of the V5-His tag following purification (BaculoDirect N-Term Linear DNA)
  • C-terminal V5-His tag for detection with Invitrogens Anti-V5 Antibody and purification with nickel-chelating resin (BaculoDirect C-Term Linear DNA)

Baculovirus Production and Titering/Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

TRUSTED_SUSTAINABILITY

Specifications

Promoter Polyhedrin
Product Type Transfection Kit
Content And Storage The BaculoDirect™ transfection kits include five 0.3 μg vials of BaculoDirect Linear DNA, 125 μL Cellfectin transfection reagent, and ganciclovir.

Store the BaculoDirect Linear DNA and Cellfectin at 4°C. All components are guaranteed stable for 6 months if stored properly.
Cleavage TEV Protease Recognition Site
Protein Tag His Tag (6x), V5 Epitope Tag
Cloning Method Gateway
Vector BaculoDirect Vectors
Product Line BaculoDirect™, Gateway™

For Research Use Only. Not for use in diagnostic procedures.

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