Invitrogen™ Bac-to-Bac™ HT Vector Kit

Designed for use as part of Bac-to-Bac Baculovirus Expression System for expression and purification of histidine-tagged recombinant proteins in Sf9, Sf21, or High Five™ Cells following bacmid generation in E. coli

Brand:  Invitrogen™ 10584027

Product Code. 10022782

Includes: The Bac-to-Bac HT Vector Kit includes 10μg of pFastBac HT vector and pFastBac HT-CAT control vector.

Description

• Strong polyhedrin promoter for protein expression
• Three reading frames for simplified cloning
• N-terminal 6 x His tag for simple purification of recombinant fusion proteins
• TEV protease cleavage site for removal of histidine tag following protein purification

Baculovirus Production and Titering, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

• Promoter: Polyhedrin
• Content And Storage: The Bac-to-Bac™ HT Vector Kit includes 10 μg of pFastBac™ HT vector and pFastBac HT-CAT control vector.
  
  Store the vectors at -20°C. All components are guaranteed stable for 6 months when properly stored.
• Protein Tag: His Tag (6x)
• Quantity: 1 kit
• Product Line: Bac-to-Bac™

• Product Type: Vector Kit
• Cleavage: TEV Protease Recognition Site
• Cloning Method: Restriction Enzyme/MCS
• Vector: pFastBac

For Research Use Only. Not for use in diagnostic procedures.