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Invitrogen™ AccuPrime™ Pfx DNA Polymerase

AccuPrime™ Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.

Brand:  Invitrogen™ 12344024

381.35 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : 50

Additional Details : Weight : 0.00454kg

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Product Code. 10472482

  • £572.00 / Each
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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

AccuPrime™ Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3´→5´ exonuclease) activity. Platinum™ anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime™ accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum™ Pfx alone.

With AccuPrime™ Pfx DNA Polymerase you will:
• Achieve greater than 26 times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 12 kb
• Minimize PCR optimization

Applications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. AccuPrime™ Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.

Specifications

Specifications

• AccuPrime Pfx DNA Polymerase (1 x 100 μL at 2.5 U/μL)
• 10X AccuPrime Pfx Reaction Mix (1 x 1 mL)
• 50 mM MgSO4 (1 x 1 mL)

Store at -20°C.
Low
Standard
Wet or Dry Ice
26X
200 Reactions
Separate Components
100 μL
Primer-probe
AccuPrime Pfx DNA Polymerase
200 Reactions
Hot-start PCR, High-fidelity PCR
Built-In Hot Start
Blunt
12 kb or less
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For Research Use Only. Not for use in diagnostic procedures.