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Invitrogen™ AccuPrime™ Pfx DNA Polymerase
AccuPrime™ Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.
Brand: Invitrogen™ 12344024
534.88 GBP valid until 2024-04-30
Use promo code "21741" to get your promotional price.
Code : 50
Additional Details : Weight : 0.00454kg
Description
AccuPrime™ Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3´→5´ exonuclease) activity. Platinum™ anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime™ accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum™ Pfx alone.
With AccuPrime™ Pfx DNA Polymerase you will:
• Achieve greater than 26 times higher fidelity than Taq DNA polymerase
• Amplify fragments up to 12 kb
• Minimize PCR optimization
Applications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. AccuPrime™ Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
Specifications
• AccuPrime Pfx DNA Polymerase (1 x 100 μL at 2.5 U/μL) • 10X AccuPrime Pfx Reaction Mix (1 x 1 mL) • 50 mM MgSO4 (1 x 1 mL) Store at -20°C. |
|
Low | |
Standard | |
Wet or Dry Ice | |
26X | |
200 Reactions | |
Separate Components | |
100 μL |
Primer-probe | |
AccuPrime Pfx DNA Polymerase | |
200 Reactions | |
Hot-start PCR, High-fidelity PCR | |
Built-In Hot Start | |
Blunt | |
12 kb or less |
For Research Use Only. Not for use in diagnostic procedures.