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Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase

High-specificity, high-yield PCR amplification of GC-rich templates

Brand:  Invitrogen™ 12337024

Code : 50

Additional Details : Weight : 0.03000kg

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Product Code. 10033322

  • £1618.00 / Each

Estimated Shipment date
from Supplier 08-06-2020

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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B).

  • Yield: high yields for targets up to 5kb in length
  • Specificity: AccuPrime accessory proteins for improved PCR specificity
  • Sensitivity: sensitivity down to 5ng of template DNA
  • Robust and specific amplifications
  • Built-in hot start

The AccuPrime buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.

Unit definition
One unit of AccuPrime GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10nmoles of dNTPs into acid-insoluble material in 30 minutes at 74°C.

Amplification of Bisulfite-Treated DNA, GC-Rich PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research

Specifications

Specifications

• 1 x 500 μL AccuPrime GC-Rich DNA Polymerase (2U/µL)
• 1 x 5 mL 5X AccuPrime GC-Rich Buffer A
• 1 x 5 mL 5X AccuPrime GC-Rich Buffer B
• 1 x 1 mL 50 mM MgSO4

Store at -20°C.

2 X
Built-In Hot Start
AccuPrime™ GC-Rich DNA Polymerase
1000 reactions
Standard
5 kb or less
3' - 5'
High
Blunt
AccuPrime™
Separate Components
Approved for shipment on Wet or Dry Ice
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For Research Use Only. Not for use in diagnostic procedures.