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Invitrogen™ 2-YT Broth
Description
2-YT Broth is a nutrient-rich microbial growth medium used for cultivation of E. coli and growth of hosts for replication of M13 vectors. The 2-YT formulation is similar to Lennox LB medium, but contains double the concentration of yeast extract and a 60% higher concentration of peptone. 2-YT medium allows cells to reach higher density and increase recombinant protein production compared to LB media formulations.
2-YT Broth formulation
Formulation per liter: 16 g SELECT Peptone 140 (pancreatic digest of casein), 10 g SELECT Yeast Extract, and 5 g NaCl.
Note: To prepare 1 L of 2-YT medium, weigh 31 g of powder and add distilled water to 1 L. Adjust the pH value to 7.0 with 5 N NaOH and sterilize by autoclaving for 20 minutes at 121°C.
Primary applications
• Growth of hosts for replication of M13 vectors
• Recombinant protein expression
• Achieve higher plasmid DNA yield
Find the media format that fits you needs
We offer a variety of ready-to-use media formulations including LB Broth (Cat. No. 10855001), Terrific Broth (Cat. No. A1374301) and S.O.C Recovery Medium (Cat. No. 15544034).
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Content And Storage | • 2-YT Broth Powder (500 g) Store at room temperature. |
| Format | Bottle |
| Media Type | 2-YT Broth (Yeast Extract Tryptone) |
| Preparation Method | Autoclave |
| Product Type | Dehydrated Culture Media |
| Target Organism Class | Coliforms |
| Quantity | 500 g |
| Shipping Condition | Room Temperature |
| Species | Escherichia coli |
For Research Use Only. Not for use in diagnostic procedures.
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