Supplied at 1μg/μL in 10mM Tris-HCl (pH 7.5), 50mM NaCl, 0.1mM EDTA
123 to 3075 bp
123 bp DNA Ladder
Prepared from a plasmid containing repeats of a 123-bp DNA fragment (1), the ladder consists of 34 fragments ranging in length from 123 to 4,182bp. Up to 25 separate bands may be clearly visualized on 0.7% to 1.5% agarose gels after ethidium bromide staining. The ladder is also useful on 4% to 8% polyacrylamide gels. This ladder can be stained with ethidium bromide or radiolabeled using T4 polynucleotide kinase, T4 DNA polymerase, DNA polymerase I, or the large fragment of DNA polymerase I (Klenow fragment). Compatible with agarose gels.
Acrylamide Gel Electrophoresis, Agarose Gel Electrophoresis, DNA and RNA Purification and Analysis, Nucleic Acid Gel Electrophoresis and Blotting