Gibco™ Insulin-Transferrin-Selenium (ITS-G) Select Supplement (100X)

Product Code.	Quantity	Unit Size
30282859	10 mL	10mL

Product Code. 30282859 Supplier Gibco™ Supplier No. A4000046501

Description

Insulin-Transferrin-Selenium (ITS-G) Select Supplement is an animal-origin-free (AOF) basal medium supplement that is used to reduce the amount of FBS needed to culture cells.

Gibco Insulin-Transferrin-Selenium (ITS-G) Select Supplement is an animal origin-free (AOF) basal medium supplement. The insulin and transferrin in the ITS-G Select formulation are produced in non-mammalian recombinant expression systems.

ITS-G is used as a basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells. Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G is now routinely used with other basal media to support a wide variety of cell types in the presence of 2–4% FBS.

Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and may also help reduce toxic levels of oxygen radicals and peroxide. Selenium, as sodium selenite, is a co-factor for glutathione peroxidase and other proteins and is used as an antioxidant in media.

The product formulation contains the following components:

• Insulin (1000.0 mg/L)
• Transferrin (550.0 mg/L)
• Sodium selenite (0.67 mg/L)

cGMP manufacturing and quality system

ITS-G Select Supplement is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

• Concentration: 100 X
• Culture Type: Mammalian Cell Culture
• Form: Liquid
• Product Type: Cell Culture Supplement
• Sterility: Sterile-filtered
• Sterilization Method: Sterile-filtered
• Endotoxin Level: ≤ 1.00 EU/mL
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Osmolality: 260 to 320 mOsm/kg
• pH: 7.8 to 9.0
• Quantity: 10 mL
• Shipping Condition: Room Temperature
• Source: Animal Origin-free

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.