Thermo Scientific™ Immuno Clear Standard Modules

Product Code.	Surface Treatment	Volume (Metric) Working	Well Shape	Well Design	unitSize
10721634	MaxiSorp	350 μL	Round, Flat Bottom (F-well)	F8	Pack of 120
10294021	PolySorp	250 μL	Curved Edges, Flat Bottom (C-well)	C8	Pack of 60
10574941	MaxiSorp	250 μL	Round, C Bottom	C8	Pack of 60
10274071	PolySorp	250 μL	Round, C Bottom	C8	Pack of 60
10172881	MaxiSorp	250 μL	Round, C Bottom	C8	Pack of 60
10032391	MediSorp	350 μL	Round, Flat Bottom (F-well)	F8	Pack of 60
10052411	MultiSorp	350 μL	Round, Flat Bottom (F-well)	F8	Pack of 60
10797921	MaxiSorp	350 μL	Round, Flat Bottom (F-well)	F16 (2x8 wells)	Pack of 60
10554831	MaxiSorp	350 μL	Round, Flat Bottom (F-well)	F8	Pack of 60
10673832	PolySorp	350 μL	Round, Flat Bottom (F-well)	F8	Pack of 60
12799995	Hydrophilic	250 μL	Round	U16	Pack of 320
10174811	MaxiSorp	350 μL	Round, Flat Bottom (F-well)	F16 (2x8 wells)	Pack of 320
10738091	PolySorp	350 μL	Round, Flat Bottom (F-well)	F16 (2x8 wells)	Pack of 320
10227541	MaxiSorp	350 μL	Round, Flat Bottom (F-well)	F8	Pack of 640
10748091	PolySorp	350 μL	Round, Flat Bottom (F-well)	F8	Pack of 640
10244551	MaxiSorp	250 μL	Round, C Bottom	C12	Pack of 60
10505411	PolySorp	250 μL	Round, C Bottom	C12	Pack of 60
10386231	MaxiSorp	250 μL	Round	U8	Pack of 60
10396231	PolySorp	250 μL	Round	U8	Pack of 60
10079074	Immulon 2 HB	330 μL	Round, Flat Bottom (F-well)	F12	Pack of 320
10406165	Immulon 2 HB	330 μL	Round, Flat Bottom (F-well)	F12	Pack of 100
10364025	Immulon 1 B	330 μL	Round, Flat Bottom (F-well)	F12	Pack of 100
10753886	Immulon 4 HBX	330 μL	Round, Flat Bottom (F-well)	F12	Pack of 100
10695757	Enhanced Binding	350 μL	Round, Flat Bottom (F-well)	F8	Pack of 50
10497305	Immulon 1 B	330 μL	Round, Flat Bottom (F-well)	F12	Pack of 320

Product Code. 10721634 Supplier Thermo Scientific™ Supplier No. 434797

Description

Perform applications that deliver well-to-well reproducibility with these immuno clear standard modules. Manufactured to fit standard equipment.

Perform applications which are delivering well-to-well reproducibility with Thermo Scientific™ Immuno Clear Standard Modules, which feature Nunc™, Immulon™ and Microtiter™ module plates developed for ELISA techniques. Manufactured to fit standard equipment, modules provide flexible use of components with small sample sizes. They are backed by industry-leading quality standards and certifications.

Thermo Scientific standard modules are strip plates with solid modules that can be easily inserted and removed from their frame. This enhances the use of the plate with smaller sample sizes.

• Several strip formats: 8-well strip, 12-well strip, 16-well strip (two columns of 8 wells)
• Several well shapes: flat-bottom, C-bottom (radius edge), U-shape wells, StarWells (wells have fins to increase the surface area and enhance performance)
• Several surfaces to maximize assay performance
• Modules remain in frame when inverted
• Stackable frame marked for lid orientation
• Polystyrene wells in polyethylene frame
• Ext. Dim.: 128 × 86mm

Choice of Surface

• PolySorp™, Immulon 1B, and Universal Binding (UB) for adsorption of hydrophobic molecules
• Medisorp™ and Immulon 2HB are slightly hydrophilic and bind a diverse range of biomolecules
• MaxiSorp™, Immulon 4 HBX, and Enhanced Binding(EB) are hydrophilic and ideal for antibody sandwich assays
• MultiSorp™ are very hydrophilic and works well for glycans and water soluble proteins

Ordering Information:

Free-standing modules. Cat. No. 95029440 and 464394 are certified. Frames and modules available separately. Sealing tape and strip caps are available. Color-coded modules are available on request. Contact your Customer Service or Sales Representative for further details.

Certifications

Products specified in chart are certified for: Adsorption of IgG; CV of <5% between wells; all results +/-10% from mean; and background of all wells +/-0.005 absorbance units from mean.

Specifications

• Breakapart: No
• Binding Property: Hydrophilic
• Certifications/Compliance: Certified for adsorption of IgG; CV of <5% between wells
• Certified: Yes
• Format: Standard
• Lid: Without Lid
• Detection Method: Colorimetric
• Array: 8 x 12
• Packaging: 120 Cases
• Binding Type: Adsorption
• Color: Clear
• Product Type: Microplate Strip
• Material: Polystyrene
• No. of Wells: 96
• Volume (Metric) Working: 350 μL
• Sterility: Non-sterile
• Surface Treatment: MaxiSorp
• Well Shape: Round, Flat Bottom (F-well)
• No. per Case: 120
• No. per Pack: 20
• Plate Blocking: None
• Quantity: 120/Cs.
• Target Molecule: Antibodies (Glycoproteins)
• Well Design: F8

Frequently Asked Questions (FAQs)

What frames do you recommend for your Immuno Clear Standard Modules?

We recommend Frames and Accessories for Immuno Breakable Modules, BreakApart frame (Cat. No. 465404). This frame is designed to hold the Immuno Clear Standard Modules.

What length of peptide is ideal for binding to the Thermo Scientific MaxiSorp surface? What are the detection limitations?

We have tested and found that a 3 amino acid peptide (Pro, Leu, Gly) cannot be detected when passively adsorbed on the MaxiSorp surface. However, this peptide can be detected when covalently immobilized using CovaLink NH Modules and CovaLink NH2 Modules and Plates. Using covalent immobilization of small peptide residues, you can typically obtain a better orientation of the molecule and reduced problems with antibody recognition of the peptide due to masking of the epitope. We have discovered that a 7 amino acid peptide from the MHC Class II antigen can be detected when adsorbed on the MaxiSorp surface. We state that the detection limitation using the MaxiSorp surface is between 3 and 7 amino acid residues.
One additional note is that detection is contingent upon the orientation of the peptide when immobilized. If the active site is inactivated or hidden at the site facing the solid phase, no detection signal is observed.

Can I bind either single- or double-stranded DNA to the Thermo Scientific MaxiSorp surface?

Single-stranded DNA can be adsorbed to our MaxiSorp surface using approximately 10 µg ssDNA per mL PBS, pH 8.2, although the stability is uncertain. Based on our experience, ssDNA immobilized on the MaxiSorp surface is so loosely bound that it can be removed by stringent washing.
Double-stranded DNA will not bind to the MaxiSorp surface. DNA, however, can be covalently bound to Nunc NucleoLink Strips.

Are your Thermo Scientific 8-Well Strip Caps for Immuno Standard Modules compatible with all Nunc MicroWell 96-Well Microplates and Nunc Immuno 96-Well Plates?

Our Thermo Scientific 8-Well Strip Caps for Immuno Standard Modules were designed to provide a positive seal for flat and round bottom wells of our Nunc MicroWell 96-Well Microplates and Nunc Immuno 96-Well Plates.
8-Well Strip Caps for Immuno Standard Modules are not compatible with C or V bottom wells of the Nunc Microwell 96-Well Microplates and Nunc Immuno 96-Well Plates.

For Nunc Immuno plates and modules, what are the advantages of one well geometry type over another? Which types should I use for various applications?

The following list describes the geometries of wells available for Nunc Immuno-plates and modules:
- Flat bottom (F): Allows maximum transmission of light. These plates can be read on a monochromatic reader.
- Round bottom (U): This geometry optimizes washing and coating. These plates can be read using a dual wavelength reader.
- "C" bottom (C): This design of the well is a combination of both flat and round bottoms. Basically, it is a flat bottomed well with curved edges at the bottom. These plates also can be read using a monochromatic reader combining optimal reading and washing.
- StarWell: These wells have a modified "C" shape geometry with eight fins strategically placed at the bottom. This feature increases surface area, allowing more molecules to become immobilized which reduces incubation times.

What is the difference between certified and non-certified MaxiSorp plates and modules?

Both of these surfaces are identical. The only difference between them is that for the certified plates, a representative sample from each manufacturing lot undergoes a Binding Capacity test. This test is an ELISA-like assay used in our quality control laboratories to ensure binding capabilities.

How can I reduce high background readings and non-specific binding, when performing ELISA using Nunc-Immuno MaxiSorp Plates?

Assay sensitivity depends strongly on efficient removal of non-specific reacting molecules. High background readings and coating instability can be minimized by addition of a blocking step after the first coating. The excess surface is then occupied by indifferent molecules.
We recommend washing three times after each coating step by using a solution of 0.15 M phosphate buffer (pH 7.2) with 0.2 M NaCl and 0.05% Tween 20.
For blocking, we recommend using 0.5% BSA, 1% casein or 1% gelatin in 0.15 M phosphate buffer (pH 8.2) or carbonate buffer (pH 9.6).

Which surfaces do you recommend for RIA (radioimmunoassay)?

The choice of plates or modules will depend on the type of RIA you are planning to perform and specific assay conditions. Nunc provides two different surfaces for immunoassays, PolySorp and MaxiSorp.
- MaxiSorp surface is highly charged and should be used if the assay requires quantitative measurement of proteins (antibodies) or molecules with polar groups.
- PolySorp surface should be used if the assay consists of less polar molecules with hydrophobic characteristics.

Which 96-well Immuno plates or modules do you recommend for various applications?

The following list offers a brief description of the features of Nunc products and their specific applications:
- Nunc-Immuno Plates, MaxiSorp surface - These plates are designed for solid phase immuno assays and have a polystyrene surface with high affinity for polar groups and hydrophilic molecules. These 96-well plates are available with flat (F), round (U), or (C) bottom-well designs.
- Nunc-Immuno Plates, PolySorp surface - These plates have a polystyrene surface which adsorps less polar molecules compared to the MaxiSorp surface and has a high affinity for hydrophobic groups. These 96-well plates are available with flat (F), round (U), or (C) bottom-well designs.
- Nunc-Immuno Modules - These modules are designed for solid-phase immuno assays. The modules are available in 8-, 12- or 16-well formats with (F), (U), and (C) bottom-well and 8-well BreakApart with (C) bottom-well designs. These different formats allow one to choose the style which is appropriate for their assay design.
BreakApart Modules and LockWell Modules can be used for radioimmunoassays.
- Nunc StarWell Immuno Breakable Modules - The 8-well modules feature eight fins on the inner wall of the (C) bottom wells. This design increases the surface area by 50%. The increase in surface area allows more molecules to be immobilized, increasing assay signal. The fin configuration provides shorter diffusion distance to the surface, reducing incubation times.
- LockWell Immuno Breakable Modules - Each plate consists of 1 x 8 breakable strips. These strips are assembled in a designed frame which locks each well into place by a spring lock. This spring lock design orientates each well at the same horizontal level allowing uniform washing and reading. The LockWell Modules are available with round (U), (C) or StarWell bottom-well designs.
- Plates and Modules with (CovaLink) Covalent Binding Surfaces: These plates can be used to bind proteins, peptides, DNA or carbohydrates. Covalent linkage occurs via secondary amine group. This allows a specific orientation and provides improved stability compared to passive adsorption due to chemical binding between molecules. Binding of molecules on PolySorp and MaxiSorp surfaces is obtained by passive adsorption. CovaLink is a surface grafted with secondary amino groups (<NH) which serves as a bridge for covalent coupling. The covalent binding can immobilize small molecules such as biotin or peptides which may otherwise bind weakly by physical adsorption and facilitate the recognition by detection molecule.
- Nunc FluoroNunc/LumiNunc 96-Well Plates: These plates are optimized for IFMA (Immunofluorometric Assays) or FIA (Fluorometric Immuno Assays). The transparent polystyrene plates give a low background fluorescence and are optimal where a read-through system is used. The white plates/modules provide maximum reflection of fluorescence signal while maintaining low background. The white plates/modules are often used for epifluorescence reading. Black modules reduce background fluorescence and minimize back-scatter light which is often encountered in epifluorescence.

What is the maximum binding capacity for proteins on the Thermo Scientific MaxiSorp surface and PolySorp surface?

Molecules bind to our PolySorp and MaxiSorp surfaces through passive adsorption.
Using IgG as a reference molecule and knowing that it is a globular molecule, theoretical calculations indicate that the maximum binding for proteins are:
- For the MaxiSorp surface, in monolayer: 650 ng/cm^2
- For the PolySorp surface: 220 ng/cm^2.

What is the difference between your Thermo Scientific PolySorp, MaxiSorp, and MiniSorp surfaces?

MaxiSorp, PolySorp and MiniSorp surfaces were developed for immunology assays:
- The MaxiSorp surface is a modified, highly charged polystyrene surface with high affinity for molecules with polar or hydrophilic groups. The surface has a high binding capacity for proteins, including globular antibodies in proper orientation. Thus, it offers very high sensitivity in double antibody "sandwich" tests.
- The PolySorp surface is more hydrophobic than the MaxiSorp surface. It has high affinity to molecules of a more hydrophobic character. This surface is particularly suited to non-protein antigens including virus antigens.
- The MiniSorp surface is a polyethylene surface with very low affinity to molecules of any type. This type of surface is ideal for the liquid phase immuno techniques.
- The MiniSorp surface is only available in a tube format, while the MaxiSorp and PolySorp surfaces are offered in both 96-well plate and module formats.

Which Nunc immunodiagnostic passive surface should I choose: PolySorp, MediSorp, MaxiSorp, or MultiSorp?

Our passive immunodiagnostic surfaces are based on a series of treatments that modify the polystyrene surface to different final levels of hydrophilicity. Selection of one of these surfaces for ELISA development should be based on the characteristics of the biomolecule you seek to immobilize. PolySorp is fairly hydrophobic and should be used for biomolecules that have hydrophobic domains, e.g., lipids, lipoproteins, and large proteins. MediSorp is slightly hydrophilic and can be used for biomolecules with hydrophilic and hydrophobic properties, e.g., medium-to-large proteins such as albumin, or amphiphilic biomolecules such as LPS. MaxiSorp is quite hydrophilic, optimized for binding of IgG (and any other molecule with similar characteristics) and thus is a popular choice for sandwich ELISA and similar protocols. MultiSorp is very hydrophilic, and in turn will immobilize very hydrophilic biomolecules such as glycoproteins. Some optimization will always be necessary, and in many cases screening for binding optimization may involve a few surface types.

What is the difference between a certified and an uncertified immunodiagnostic plate, e.g., Cat. No. 439454 vs. 442404?

There is no difference in the manufacturing process. Certified plates are performance tested (using an antibody binding protocol) during quality control, whereas uncertified plates are not. This test is expensive for us to perform, so we offer the uncertified version at cost savings customers for whom antibody binding testing results are not critical.