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Thermo Scientific™ Immuno Clear Standard Modules
Description
Perform applications which are delivering well-to-well reproducibility with Thermo Scientific™ Immuno Clear Standard Modules, which feature Nunc™, Immulon™ and Microtiter™ module plates developed for ELISA techniques. Manufactured to fit standard equipment, modules provide flexible use of components with small sample sizes. They are backed by industry-leading quality standards and certifications.
Thermo Scientific standard modules are strip plates with solid modules that can be easily inserted and removed from their frame. This enhances the use of the plate with smaller sample sizes.
- Several strip formats: 8-well strip, 12-well strip, 16-well strip (two columns of 8 wells)
- Several well shapes: flat-bottom, C-bottom (radius edge), U-shape wells, StarWells (wells have fins to increase the surface area and enhance performance)
- Several surfaces to maximize assay performance
- Modules remain in frame when inverted
- Stackable frame marked for lid orientation
- Polystyrene wells in polyethylene frame
- Ext. Dim.: 128 × 86mm
Choice of Surface
- PolySorp™, Immulon 1B, and Universal Binding (UB) for adsorption of hydrophobic molecules
- Medisorp™ and Immulon 2HB are slightly hydrophilic and bind a diverse range of biomolecules
- MaxiSorp™, Immulon 4 HBX, and Enhanced Binding(EB) are hydrophilic and ideal for antibody sandwich assays
- MultiSorp™ are very hydrophilic and works well for glycans and water soluble proteins
Ordering Information:
Free-standing modules. Cat. No. 95029440 and 464394 are certified. Frames and modules available separately. Sealing tape and strip caps are available. Color-coded modules are available on request. Contact your Customer Service or Sales Representative for further details.
Certifications
Products specified in chart are certified for: Adsorption of IgG; CV of <5% between wells; all results +/-10% from mean; and background of all wells +/-0.005 absorbance units from mean.
Specifications
Specifications
| Breakapart | No |
| Binding Property | Slightly Hydrophilic |
| Certifications/Compliance | Certified for adsorption of IgG; CV of <5% between wells |
| Certified | Yes |
| Format | Strip Plate |
| Lid | With Lid |
| Detection Method | Colorimetric |
| Packaging | Inner packs of 10 |
| Binding Type | Adsorption |
| Color | Clear |
| Show More |
Frequently Asked Questions (FAQs)
We recommend Frames and Accessories for Immuno Breakable Modules, BreakApart frame (Cat. No. 465404). This frame is designed to hold the Immuno Clear Standard Modules.
Our Thermo Scientific 8-Well Strip Caps for Immuno Standard Modules were designed to provide a positive seal for flat and round bottom wells of our Nunc MicroWell 96-Well Microplates and Nunc Immuno 96-Well Plates.
8-Well Strip Caps for Immuno Standard Modules are not compatible with C or V bottom wells of the Nunc Microwell 96-Well Microplates and Nunc Immuno 96-Well Plates.
The following list describes the geometries of wells available for Nunc Immuno-plates and modules:
- Flat bottom (F): Allows maximum transmission of light. These plates can be read on a monochromatic reader.
- Round bottom (U): This geometry optimizes washing and coating. These plates can be read using a dual wavelength reader.
- "C" bottom (C): This design of the well is a combination of both flat and round bottoms. Basically, it is a flat bottomed well with curved edges at the bottom. These plates also can be read using a monochromatic reader combining optimal reading and washing.
- StarWell: These wells have a modified "C" shape geometry with eight fins strategically placed at the bottom. This feature increases surface area, allowing more molecules to become immobilized which reduces incubation times.
Our passive immunodiagnostic surfaces are based on a series of treatments that modify the polystyrene surface to different final levels of hydrophilicity. Selection of one of these surfaces for ELISA development should be based on the characteristics of the biomolecule you seek to immobilize. PolySorp is fairly hydrophobic and should be used for biomolecules that have hydrophobic domains, e.g., lipids, lipoproteins, and large proteins. MediSorp is slightly hydrophilic and can be used for biomolecules with hydrophilic and hydrophobic properties, e.g., medium-to-large proteins such as albumin, or amphiphilic biomolecules such as LPS. MaxiSorp is quite hydrophilic, optimized for binding of IgG (and any other molecule with similar characteristics) and thus is a popular choice for sandwich ELISA and similar protocols. MultiSorp is very hydrophilic, and in turn will immobilize very hydrophilic biomolecules such as glycoproteins. Some optimization will always be necessary, and in many cases screening for binding optimization may involve a few surface types.
There is no difference in the manufacturing process. Certified plates are performance tested (using an antibody binding protocol) during quality control, whereas uncertified plates are not. This test is expensive for us to perform, so we offer the uncertified version at cost savings customers for whom antibody binding testing results are not critical.
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