Thermo Scientific™ Imject™ Maleimide-Activated BSA and Kit

Prepare hapten-carrier conjugates with cysteine-containing peptides and other thiolated antigens using this sulfhydryl-reactive bovine serum albumin.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 77115

10 MG MALEIMIDE ACTIVATED BSA, IMJECT 10MG

UNSPSC: 41106500

Code: PN

Additional Details:
Additional Details: CAS Number: 67423-45-4 Weight: 0.14000kg



Product Code. 10648304

Quantity Price
1 £ 299.0 / 10mg
Estimated Shipment date
from Supplier 03-02-2017
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Description and Specification

Specification

Formulation Activated protein lyophilized in 0.1M sodium phosphate, 150mM sodium chloride, 0.1M EDTA, 10mM sucrose, pH 7.2
Sufficient For 5 conjugation reactions, each with up to 2mg sulfhydryl-containing peptide
Format Lyophilized
Description Imject Maleimide Activated Bovine Serum Albumin
Protein BSA, bovine serum albumin
Activation Maleimide
Quantity 10mg

Purified, carrier-grade BSA is rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated protein is then stabilized and lyophilized in phosphate buffer. Maleimide groups form covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other thiol-containing haptens. Several antigen molecules can be conjugated to each BSA molecule to increase their immunogenicity and likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest. Select from among several package sizes of activated BSA protein, or choose the complete conjugation kit that includes optimized buffers and trouble-free spin desalting columns.

Highlights:

  • Convenient packages – choose stabilized, lyophilized Maleimide-Activated BSA or complete immunogen conjugation kit

  • Optimized activation – specifications require that each lot is activated with 15 to 20 moles of sulhydryl-reactive maleimide per mole of BSA, ensuring consistently high levels of conjugation

  • Sulfhydryl-reactive – engineered for conjugating cysteine-containing peptide antigens to make effective immunogens for high-titer antibody production

  • Simple, rapid procedure – complete the hapten-carrier conjugation reaction in two hours to yield sufficient immunogen for several injections and booster immunizations of host animals

  • High-quality carrier protein – pure BSA (Fraction V) is activated using high-quality Thermo Scientific Sulfo-SMCC crosslinker and formulated to ensure high stability and consistent performance

  • Immunogenic and soluble – moderately immunogenic and highly soluble, BSA is useful for preparation of the primary immunogen or as an irrelevant hapten-carrier for antibody screening and immunoassays after using KLH as the carrier protein to generate the immune response against the hapten

Includes:

Kit contains stabilized, lyophilized Maleimide-Activated BSA (in PBS with stabilizer), buffers and desalting columns to easily prepare ready-to-use immunogens for immunization