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Cytiva Ready-To-Go™ GenomiPhi V3 DNA Amplification Kit

Highly efficient and representative whole-genome amplification with 40 to 60μg yield from nanogram amounts of DNA sample. Cytiva Lifescience™ illustra™ Ready-To-Go™ GenomiPhi V3 DNA Amplification Kit is part of the Phi29 DNA polymerase family of products.

Brand:  Cytiva 25-6601-96

Code : GH

Additional Details : Weight : 0.10000kg

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Product Code. 12967160

  • £538.00 / Each
Estimated Shipment: 18-04-2024
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Description

Description

 

  • Representative amplification of the whole genome.
  • Preformulated, predispensed, single-dose, lyophilized cakes for rapid reaction set-up and greater reproducibility.
  • Quick and simple automation-friendly protocol; no thermal cycler required.
  • Shipping and storage at room temperature provides easy access and reduced carbon footprint.
  • Yields high-quality DNA (e.g. for high-throughput genotyping and DNA archival).
  • The reaction mixture is provided as predispensed, single-dose, lyophilized cakes in either strips of 8 tubes, or 96-well plates.
  • Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample.
  • Amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity.
  • The average product length is over 10kb.
  • Procedure is quick, simple and does not require a thermocycler.
  • A typical DNA yield of 12 to 20μg DNA can be achieved in less than two hours from only 10ng of genomic DNA input.
  • The starting material for GenomiPhi reactions can be purified DNA from any commercial kit or homebrew method, or a nonpurified cell lysate may be used.
  • Protocols for the amplification of DNA from blood lysates and dried blood spots are included in the manual.
  • GenomiPhi amplified DNA is suitable for various applications such as genotyping (SNP, STR, array CGH), cloning, sequencing, and DNA archival.

 

Specifications

Specifications

For whole genome amplification by isothermal strand displacement amplification; typical downstream applications are genotyping (SNP, STR, array CGH) and DNA archiving.
Ready-To-Go GenomiPhi V3 cakes, 1 × 96-well plate; 2x Denaturation buffer, 1 × 1.2 ml; Control DNA (Lambda), 500 ng; 12 Strips of 8 domed caps
96
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Safety and Handling

Safety and Handling

Hazard Statement
  • EUH210-Safety data sheet available on request.

Supplemental information
  • UNKNOWN ACUTE-72.5 per cent of the mixture consists of component(s) of unknown acute toxicity.
  • UNKNOWN AQUATIC-Contains 72.5% of components with unknown hazards to the aquatic environment.

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