Cytiva PlasmidPrep Mini Spin Kit

Product Code. 11399941

Brand: Cytiva 28904271

Description

Purify high yields of plasmid DNA in less than 10 minutes

Purify high yields of plasmid DNA in less than 10 minutes. Color-coded tubes and columns simplify the protocol and purified DNA is suitable for all common downstream applications. illustra plasmidPrep Mini Spin Kit uses a simple plasmid DNA purification protocol involving a modified alkaline lysis procedure and a novel silica-based membrane to achieve highly efficient plasmid DNA purification. The typical plasmid DNA yield from a fresh 1.5mL culture of E. coli containing a high copy number plasmid is approximately 9μg. Instead of organic solvents, the kit contains chaotropic salts to denature protein components and promote selective binding of DNA to the novel silica membrane. The kit contains spin columns prepacked with a novel silica membrane, suspension, lysis, and neutralisation buffers containing chaotropic salts, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.

• Total preparation time of less than 10 minutes represents a 50% reduction in protocol time.
• Produces high-quality plasmid DNA with excellent reproducibility for use in cloning, restriction enzyme digestion, PCR amplification, and DNA sequencing.
• Increased ease of use provided by minimal changes in pipetting volume from one part of the protocol to the next, fewer centrifugation steps, color-coded kit components, and lack of organic solvents.

Specifications

• Description: Purify high yields of plasmid DNA in less than 10 min. Color-coded tubes and columns simplify the protocol and purified DNA is suitable for all common downstream applications.
• For Use With (Application): Suitable for many downstream applications including—restriction enzyme analysis, ligation, cloning, DNA sequencing and PCRSequencing, PCR, ligation and cloning, restriction digest, enzymatic modification, template for labeled probe generation, transforma
• Product Type: illustra Plasmid Mini Spin
• Quantity: 10 Pack
• Purity: 1.7
• Sample Volume: 3.0 mL
• Test Time: Approx. 9 min
• Yield: 6 to 15μg

Safety and Handling

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2