Cytiva Blood Genomic Prep Mini Spin Kit

Product Code. 11379941

Brand: Cytiva 28904263

Description

illustra blood genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA)

illustra blood genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from whole blood, buffy coat, bone marrow, and nucleated red blood cells. The purification process involves minimal shearing, resulting in the production of 5 to 10 μg of good quality, intact genomic DNA from a 200μL sample. Input sample volumes can vary from 50μL to 1mL. The method uses a simple genomic DNA purification protocol that uses chaotropic agents to extract DNA from blood cells, denature protein components, and promote the selective binding of DNA to a column-based, novel silica membrane. The elution volume can be modified as necessary to obtain the appropriate genomic DNA concentration for most downstream molecular biological applications. The kit contains spin columns prepacked with a novel silica membrane, lyophilised proteinase K powder, lysis solution, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.

• For the rapid and reproducible isolation of high-quality genomic DNA from whole blood, buffy coat, bone marrow, and nucleated red blood cells.
• High-quality genomic DNA with a greater proportion of higher molecular weight DNA and less shearing.
• Demonstrated application to various types of whole blood including human, horse, rabbit, rat, and mouse.
• Increased ease of use with reduced pipetting volume changes, one centrifugation setting, and color-coded kit components
• Verified in several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests.

Specifications

• Description: illustra blood genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from whole blood, buffy coat, bone marrow, and nucleated red blood cells.
• For Use With (Application): Used for several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digestsSuitable for use in molecular biology applications including standard, multiplex and quantitative PCR; cloning; haplotyping; rest
• Product Type: illustra Blood Mini Spin (10)
• Quantity: 10 Pack
• Purity: 1.9
• Sample Volume: 300 μl whole blood, Buffy coat, bone marrow cells, 10 μl nucleated blood†, 1000 μl whole blood‡
• Test Time: < 20 min
• Yield: 4 to 6μg/200μL whole blood

Safety and Handling

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 1
• Skin corrosion/irritation Category 2
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H302-Harmful if swallowed.
• H318-Causes serious eye damage.
• H315-Causes skin irritation.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P273-Avoid release to the environment.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P310-Immediately call a POISON CENTER/doctor/.
• P501-Dispose of contents/container to .