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Invitrogen™ IL-8 Human Uncoated ELISA Kit

Uncoated ELISA Kit

£368.00 - £524.00

Specifications

Accession Number P10145
Assay Range 2-250 pg/mL
Assay Sensitivity 2 pg/mL
Conjugate Biotin
Product Type ELISA Kit
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Product Code Brand Quantity Price Quantity & Availability  
15541197
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Invitrogen™
88-8086-77
20 x 96 Tests
£524.00
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15561197
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Invitrogen™
88-8086-88
10 x 96 Tests
£368.00
Pack of 10
In Stock
to see stock.
Edge
Add to basket
 
Description

Description

The Human Interleukin 8 (IL-8) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-8. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL-8 (Interleukin-8) is a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.
Specifications

Specifications

P10145
2 pg/mL
ELISA Kit
Human
Colorimetric Microplate Reader
GMCSF
HRP
Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
Interleukin-8, CXCL8, NAP-I
1 hr. 45 min.
2-250 pg/mL
Biotin
Plasma, Serum, Supernatant
ELISA
3576
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X Concentrated, Detection Enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards., 96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86)
RUO
2°C to 8°C
Human
24 hr. 30 min.
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