PeproTech IL-4 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Product Code	Brand	Quantity
17875433	PeproTech 500-P24BT-25UG	25 μg
17824973	PeproTech 500-P24BT-50UG	50 μg
17885073	Invitrogen 500-P24BT-1MG	2 x 500μg
30054434	PeproTech 500-P24BT-500UG	500 μg

Description

AA Sequence of recombinant protein: MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-4. Anti-Human IL-4-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hIL-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-4 (500-P24) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIL-4. Western Blot: To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P24BT-1MG will be provided as 2 x 500 μg

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

Specifications

• Antigen: IL-4
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: P05112
• Gene ID (Entrez): 3565
• Immunogen: E.coli-derived Recombinant Human IL-4.
• Purification Method: Antigen affinity chromatography
• Gene: Il4

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: B cell growth factor 1; B_cell stimulatory factor 1; B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; cytokine; H-IL-4; IGG1 induction factor; IL2; IL4; Il-4; Il4e12; ILN; Interleukin; interleukin 4; interleukin 4 variant 2; interleukin 4 variant IL-4delta2; interleukin 4 variant IL-4delta3; interleukin 4 variant IL-4int2A; interleukin 4 variant IL-4int3A; interleukin 4 variant IL-4int3B; Interleukin4; interleukin-4; interleukin-4 precursor; lymphocyte stimulatory factor 1; MGC79402; M-IL-4; Pitrakinra
• Gene Symbols: Il4
• Primary or Secondary: Primary
• Content And Storage: -20°C