Invitrogen™ Human IL-31 ELISA Kit

Product Code. 17649306

Brand: Invitrogen™ BMS20412

Description

ELISA

The Human Interleukin-31 (Hu IL-31) ELISA quantitates Hu IL-31 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-31. Principle of the method The Human IL-31 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin-31 (IL-31) is a recently discovered T-cell cytokine closely related to IL-6 type cytokines and is preferentially produced by T helper type 2 cells. IL-31 activity is mediated through the ligand-induced oligomerization of a dimeric receptor complex containing IL-31 receptor A and oncostatin M receptor. In response to IL-31 binding, these proteins activate the JAK/STAT and the AKT signaling pathways. RNA levels of IL-31 receptor A and oncostatin M receptor are induced in activated monocytes but are expressed constitutively in epithelial cells. IL-31, when overexpressed in transgenic mice, results in the development of pruritis, alopecia, and skin lesions and in humans may result in atopic dermatitis, suggesting that IL-31 may represent a novel target for antipruritic drug development.

Specifications

• Accession Number: Q6EBC2
• Assay Range: 7.8-500 pg/mL
• Assay Sensitivity: 1.2 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 1700013B14Rik; IL31; IL-31; ILN; Interleukin; interleukin 31; interleukin-311700013B14Rik; IL31; IL-31; ILN; Interleukin; interleukin 31; interleukin-31;
• Gene ID (Entrez): 386653
• Gene Symbol: Il31
• Interassay CV: 3.3%
• Intraassay CV: 5.7%
• Kit Contents: Pre-coated 96 well plate, Standard (2 vials), Sample diluent, Biotinylated Detection Antibody, Assay buffer Concentrate, Wash buffer concentrate,SAV-HRP,TMB, Stop solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein: IL-31
• Protein Aliases: IL-31, Interleukin-31
• Quantity: 96 Tests
• Regulatory Status: RUO
• Shipping Condition: Wet Ice
• Storage Requirements: 2-8°C
• Target: IL-31
• Target Species Validated: Human
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 30 min
• UniProt ID: Q6EBC2