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Invitrogen™ IL-27 p28 Polyclonal Antibody

Product Code. 12858402
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Quantity:
100 μg
Unit Size:
100µg
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12858402 100 μg 100µg
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Product Code. 12858402 Supplier Invitrogen™ Supplier No. PA520241

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Description

Rabbit Polyclonal Antibody

A suggested positive control is Daudi cell lysate. PA5-20241 can be used with blocking peptide PEP-0360.

IL-27, a member of the IL-12 family, is a heterodimeric protein consisting of the p40-related protein Epstein-Barr virus-induced gene 3 (EBI3) non-covalently linked to an IL-12p35-related protein, p28 (also known as IL-30). IL-27 is produced by activated APCs and mature dendritic cells. IL-27 exerts its activities on NK cells and naive CD4+ T cells; mRNA expression analysis of IL-27 receptor components (WSX-1/TCCR and gp130) suggests that IL-27 may also target other cells, including mast cells and monocytes. Binding of IL-27 to WSX-1/gp130 activates JAK1, STAT1, and STAT3 and STAT1/3 phosphorylation. WSX-1/TCCR-deficient mice develop impaired Th1 responses and are more susceptible to infection with L. monocytogenes suggesting that Th1 responses require IL-27. Although activation of WSX-1 is required for the initiation of Th1 responses, it is not necessary for maintaining Th1 responses. IL-27 alone is not able to induce the differentiation of CD4+ T cells into IFN-γ-producing cells, suggesting a role for IL-27 as an initial activator of Th1 responses. An important effect of IL-27 in initiating Th1 responses is the induction of the Th1-specific transcription factor T-bet as well as the suppression of the Th2-specific transcription factor GATA-3. T-bet plays a critical role in Th1 differentiation by its ability to maintain IL-12Rβ2 expression following CD4+ T cell activation. Recent studies indicate that IL-27 has a potent antitumor activity. In vitro, IL-27 has been found to act directly on naive CD8 cells, generating CTL with enhanced granzyme B expression. In vivo, IL-27 has been reported to augment CTL activity, inhibit tumor growth, and induce complete regression of primary and metastatic neuroblastoma tumors.
TRUSTED_SUSTAINABILITY

Specifications

Antigen IL-27 p28
Applications Western Blot
Classification Polyclonal
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.02% sodium azide
Gene Il27
Gene Accession No. Q8K3I6, Q8NEV9
Gene Alias IL27; IL-27; IL-27 p28 subunit; IL-27 subunit alpha; Il27a; IL-27A; IL-27-A; IL27-A; IL27p28; IL-27p28; IL30; IL-30; ILN; Interleukin; interleukin 27; interleukin 30; Interleukin-27; interleukin-27 subunit alpha; Interleukin-30; p28
Gene Symbols Il27
Host Species Rabbit
Immunogen A 14 amino acid peptide from near the center of human IL-27.
Purification Method Antigen Affinity Chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 246778, 246779
Target Species Human, Mouse
Content And Storage Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Product Type Antibody
Form Liquid
Isotype IgG
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