Invitrogen™ IL-27 Monoclonal Antibody (355028)

Rat Monoclonal Antibody

Brand:  Invitrogen™ MA524101

Product Code. 15543792

Description

In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-27 p28, rmIL-27 EBI-3, or recombinant human (rh) IL-27 heterodimer is observed. In Western blots, no cross-reactivity with rmIL-27 p28 or rhIL-27 heterodimer is observed. Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.

IL-27 (Interleukin 27) is a cytokine belonging to the IL-12 family, and is a heterodimeric cytokine composed of EBI3 and p28 subunits. IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines. IL-27 promotes CD4+ T cell differentiation to the Th1 lineage by inducing expression of the transcription factor T-bet and up regulating IL-12R beta2, which in turn suppresses Th2 and Th17 differentiation and proliferation. IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. IL-27 acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting IL-27 involvement in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. In humans, the gene is located on the p arm of chromosome 16.

Specifications

• Antigen: IL-27
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: O35228, Q8K3I6
• Gene Symbols: Ebi3, IL27
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL-27 heterodimer Tyr19-Pro228 (EBI3) and Phe29-Ser234 (p28).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Immunoprecipitation
• Clone: 355028
• Conjugate: Unconjugated
• Gene: Ebi3
• Gene Alias: cytokine receptor; EBI3; EBI-3; EBV-induced gene 3 protein; Epstein-Barr virus induced 3; Epstein-Barr virus induced gene 3; Epstein-Barr virus induced gene 3 (similar to Interleukin-27 beta chain precursor); epstein-Barr virus-induced gene 3 protein; Epstein-Barr virus-induced gene 3 protein homolog; Il27; IL-27; IL27 p28; IL-27 p28 subunit; IL27 subunit; IL-27 subunit alpha; IL-27 subunit beta; Il27a; IL-27A; IL-27-A; IL27-A; Il27b; IL-27B; IL27p28; IL-27p28; Il30; IL35 subunit; ILN; Interleukin; interleukin 27; interleukin 30; interleukin-27 subunit alpha; Interleukin-27 subunit beta; interleukin-30; MGC71873; M-IL-27 (p28); p28; RGD1561420
• Host Species: Rat
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Gene ID (Entrez): 246779, 50498
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized