IL-23 p19, Alexa Fluor 488, clone: fc23cpg, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 53-7023-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: This fc23cpg monoclonal antibody reacts with the p19 subunit of mouse IL-23, a heterodimeric cytokine made up of two disulfide-linked subunits, p40 and p19. It is closely related to IL-12, with which it shares the p40 subunit. IL-23 signals through a heterodimeric receptor expressed on activated T cells, macrophages, and dendritic cells. The receptor shares the IL-12Rbeta1 chain with IL-12, while the IL-23R chain is unique to the IL-23 receptor complex. Binding of IL-23 to its receptor activates Jak/STAT pathways similarly to IL-12, but the two cytokines exhibit distinct functions. Unlike IL-12, which induces T-bet and Th1 differentiation, IL-23 signaling results in RORgammat expression and supports CD4^+ T cells with the Th17 phenotypeDendritic cells and macrophages produce IL-23 in response to TLR2, TLR4, and TLR8 ligands, as well as agonists of the beta-glucan receptor, Dectin-1. Our studies suggest donor, kinetics and type of stimulant can result in variation in IL-23 expression levels. Recent publications suggest the p19 subunit may also exist in the absence of association with p40.Applications Reported: This fc23cpg antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This fc23cpg antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse bone marrow-derived dendritic cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|IL-23, IL-23A, IL23P19, MGC79388, P19, SGRF|
|Alexa Fluor 488|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.