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IL-2, Functional Grade, clone: JES6-1A12, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-7022-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15208747

  • £177.83 / 500µg


EU Stock 14
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Description

Description

Description: The JES6-1A12 antibody reacts with mouse interleukin-2 (IL-2). The JES6-1A12 antibody is a neutralizing antibody. Mouse IL-2 (also known as TCGF) is a 17kDa factor produced mainly by activated CD4+ T cells. IL-2 induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells. IL-2 also acts on activated B cells, monocytes, NK cells, LAK cells, and on oligodendroglial cells in vitro. In addition, IL-2 plays a role in hematopoiesis, tumor surveillance and anti-inflammatory reactions, and hence is a central regulator of the immune response. Non-glycosylated IL-2 is biologically active.Applications Reported: The JES6-1A12 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization of IL-2 bioactivity, and IP.Applications Tested: The Functional Grade Purified JES6-1A12 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-2 bioactivity. The JES6-1A12 antibody at 0.250 μg/mL has been found to inhibit by 50% the biological effects of 1.0 ng/mL mouse IL-2 in a CTLL-2 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section. The JES6-1A12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-2 (IL-2) in combination with the biotin JES6-5H4 (13-7021) antibody for detection and recombinant mouse IL-2 (14-8021) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

IL-2
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P04351
Interleukin-2, T-cell growth factor, TCGF, Aldesleukin
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Immunoprecipitation
JES6-1A12
Functional Grade
Il2
Purified
Il2
IgG2a, kappa
500μg
Store at 2-8°C.
Primary
16183
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