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PeproTech IL-12 p70 Polyclonal Antibody, PeproTech®, Invitrogen™
Description
AA Sequence of recombinant protein: p35 subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS. Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine IL-12. Anti-Murine IL-12-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-12 matrix. Sandwich ELISA: To detect Murine IL-12 by sandwich ELISA (using 100 uL/well antibody solution) a concentration of 0.5-2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-12 (500-P155GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-12. WB: To detect Murine IL-12 by WB analysis this antibody can be used at a concentration of 0.1-0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-12 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Specifications
Specifications
| Antigen | IL-12 p70 |
| Applications | ELISA, Western Blot |
| Classification | Polyclonal |
| Concentration | 0.1-1.0 mg/mL |
| Conjugate | Unconjugated |
| Formulation | PBS with no preservative |
| Gene | IL12A |
| Gene Accession No. | P43431, P43432 |
| Gene Alias | 20C2; CLMF; CLMF p35; CLMF p40; CLMF2; cytokine; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12p70; Il12; IL-12; Il-12 p35; IL-12 p75; IL-12 subunit p35; IL-12 subunit p40; IL-12, subunit p35; IL12, subunit p40; Il-12; cytokine; IL12A; IL-12A; IL12A/B; IL12AB; IL12B; Il-12b; IL12P35; IL-12p35; Il12p40; Il-12p40; IL35 subunit; ILN; IMD28; IMD29; Interleukin; interleukin 12 35 kDa subunit; interleukin 12 35kDa subunit; interleukin 12 40 kDa subunit; interleukin 12 40kDa subunit; interleukin 12 p35 subunit; interleukin 12 p40 subunit precursor; interleukin 12 polypeptide 35; interleukin 12, p35; interleukin 12, p40; interleukin 12A; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin 12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin 12p35 subunit precursor; interleukin-12 alpha chain; interleukin-12 alpha p40 subunit; interleukin-12 beta chain; interleukin-12 beta p35 subunit; interleukin-12 p35 subunit; interleukin-12 p35 subunit precursor; interleukin-12 p40; interleukin-12 p40 subunit; interleukin-12 p40 subunit precursor; Interleukin-12 p70; interleukin-12 subunit alpha; Interleukin-12 subunit beta; interleukin-23 p40 subunit; Ll12a; MGC151228; MGC151232; natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35; natural killer cell stimulatory factor, 40 kD subunit; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NK cell stimulatory factor chain 2; NKSF; NKSF1; NKSF2; P35; p40 |
| Gene Symbols | IL12A, Il12b |
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