IL-10, eFluor 450, clone: JES3-9D7, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 48-7108-41
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).Applications Reported: This JES3-9D7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This JES3-9D7 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Th2 polarized human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|Interleukin-10, CSIF, IL-10, IL10A, TGIF|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.