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IL-10, eFluor 450, clone: JES3-9D7, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-7108-41

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15278867

  • £82.98 / 25 tests


EU Stock 5
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Disclaimers:

For Research Use Only.


Description

Description

Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).Applications Reported: This JES3-9D7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This JES3-9D7 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Th2 polarized human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IL-10
JES3-9D7
eFluor 450
Conjugated
Rat
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry (Intracellular Staining)
5μL (1μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Interleukin-10
IgG1
RUO
Primary
Human
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