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Invitrogen™ IL-10 Monoclonal Antibody (9D7)
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Product Code. 11874251
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Quantity:
500 μg
Unit Size:
500µg
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11874251 500 μg 500µg
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Product Code. 11874251 Supplier Invitrogen™ Supplier No. M010

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Description

Rat Monoclonal Antibody

M010 targets IL-10 in ELISA, and IA applications and shows reactivity with Human samples. The M010 immunogen is recombinant human IL-10 (COS cell-derived). M010 detects IL-10 which has a predicted molecular weight of approximately 19 kDa. The M010 IL10 antibody (clone 9D7) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M011B (biotinylated conjugate of clone 12G8). Typical dilutions for sandwich ELISA include 1 μg/mL for coating and 0.125 - 0.25 μg/mL for detection. Antibody M010 (clone 9D7) and biotinylated antibody M011B (clone 12G8) have successfully been used in combination with recombinant IL10 protein SIL10 in ELISA applications. This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified. This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
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Specifications

Antigen IL-10
Applications ELISA, Inhibition Assays, Western Blot
Classification Monoclonal
Clone 9D7
Concentration 1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with no preservative
Gene IL10
Gene Accession No. P22301
Gene Alias CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF
Gene Symbols IL10
Host Species Rat
Immunogen Recombinant human IL-10 (COS cell-derived).
Purification Method Protein G
Quantity 500 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 3586
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Liquid
Isotype IgG1
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