Novus Biologicals™ IL-1 RI Antibody Pair [HRP]

Product Code. 18205158

Brand: Novus Biologicals™ NBP27960415PLATES

Description

Polyclonal/Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes an antibody specific for Mouse IL-1 RI.

Specifications

• Assay Range: 62.5 pg/mL
• Assay Sensitivity: 62.5-4000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Mouse IL-1 RI.
• Target Species: Mouse
• For Use With (Application): Sandwich ELISA
• Gene Alias: CD121 antigen-like family member A, CD121a antigen, IL1R, IL1R1, IL1RT1, IL-1RT1, IL-1RT-1, Interleukin 1 Receptor 1, interleukin 1 receptor, type I, interleukin receptor 1, interleukin-1 receptor type 1, p80
• Gene ID (Entrez): 3554
• Gene Symbol: IL1R1
• Host Species: Rabbit
• Kit Contents:
  Standard: Each vial contains 175 ng of recombinant Mouse IL-1 RI.
  Capture Antibody: 0.4 mg/mL of rabbit anti-Mouse IL-1 RI monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of rabbit anti-IL-1 RI polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cancer, Cytokine Research, Immunology, Innate Immunity, Phospho Specific
• Storage Requirements: Storage is content dependent.
• Target: IL-1 RI
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended.