IL-1 beta Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Goat Polyclonal Antibody

Brand:  Invitrogen 500-P21BGBT-1MG

Product Code. 17835553

Description

AA Sequence of recombinant protein: APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human IL-1beta. Anti-Human IL-1beta-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hIL-1beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-1beta (500-P21BG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIL-1beta. Western Blot: To detect hIL-1beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-1beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P21BGBT-1MG will be provided as 2 x 500 μg

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.

Specifications

• Antigen: IL-1 beta
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: IL1B
• Gene Alias: catabolin, cytokine, Hematopoietin 1 (H1), IFN beta inducing factor, il 1b, IL 1β, IL-1, IL1 B, IL-1 beta, IL-1 precursor, IL1B, IL-1B, IL1B1, IL1beta, IL-1beta, IL1-BETA, IL1F2, IL1β, ILN, Interleukin, interleukin 1 beta, Interleukin 1 beta precursor, interleukin 1, beta, interleukin 1, beta 1, interleukin 1-beta, interleukin*1*beta, Interleukin1 beta, interleukin-1 beta, interleukin-1 beta precursor, interleukin-1 beta precursor (AA -113 to 153), interleukin-1 beta proprotein, interleukin-1b, interleukin-1beta, interleukin-beta, LAF, lymphocyte proliferation-potentiating factor, Osteoclast activating factor (OAF), preinterleukin 1 beta, Pro interleukin 1 beta, prointerleukin-1 beta, pro-interleukin-1-beta
• Host Species: Goat
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3553
• Content And Storage: -20°C

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P01584
• Gene Symbols: IL1B
• Immunogen: E.coli-derived Recombinant Human IL-1beta
• Quantity: 2 x 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Form: Lyophilized