Invitrogen™ Mouse IL-1 alpha ELISA Development Kit (ABTS), PeproTech®

Product Code. 17869005

Brand: Invitrogen™ 900K82

Description

ELISA

IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Specifications

• Accession Number: P01582
• Assay Range: Lot-specific
• Conjugate: HRP
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 16175
• Gene Symbol: Il1a
• Kit Contents: Capture Antibody, Detection Antibody, Protein Standard, HRP-conjugate
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: IL-1 alpha
• Test Time: 1 hrs 45 mins
• Total Assay Time: 24 hrs 30 mins