Invitrogen™ IKK beta Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA5120783

Product Code. 17712184

Description

Positive test controls include: HeLa, A-549, Jurkat, HepG2, THP-1. The target is usually found in the following locations: Cytoplasm, Membrane raft, Nucleus. Immunogen sequence: QISPRPQPES VSCILQEPKR NLAFFQLRKV WGQVWHSIQT LKEDCNRLQQ GQRAAMMNLL RNNSCLSKMK NSMASMSQQL KAKLDFFKTS IQIDLEKYSE QTEFGITSDK LLLAWREMEQ AVELCGRENE VKLLVERMMA LQTDIVDLQR SPMGRKQGGT LDDLEEQARE LYRRLREKPR DQRTEGDSQE MVRLLLQAIQ SFEKKVRVIY TQLSKTVVCK QKALELLPKV EEVVSLMNED EKTVVRLQEK RQKELWNLLK IACSKVRGPV.

Phosphorylation of serine residues on the I-kappa-B proteins by IKK kinases, including IKBKB, marks them for destruction via the ubiquitination pathway, allowing activation of the NF-kappa-B complex. Inhibitor of nuclear factor kappa-B kinase subunit beta (IKK beta) is a serine kinase that plays an essential role in the NF-kaapa-B signaling pathway which is activated by mulitple stimuli such as inflammatory cytokines, bacterial, or viral products, DNA damages, or other cellular stresses. IKK beta acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Mutations affecting the IKK beta gene can result in Immunodeficiency 15 (IMD15).

Specifications

• Antigen: IKK beta
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Ikbkb
• Gene Alias: AI132552; AIM-1; FLJ40509; I-kappa-B kinase 2; I-kappa-B-kinase beta; Ikbkb; IKK[b]; IKK2; IKK-2; IKKB; IKK-B; IKKbeta; IKK-beta; IMD15; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of kappaB kinase beta; inhibitor of nuclear factor kappa B kinase beta subunit; inhibitor of nuclear factor kappa-B kinase subunit beta; MGC131801; NFKBIKB; nuclear factor NF-kappa-B inhibitor kinase beta
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16150, 3551, 84351
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 10.45 mg/mL
• Formulation: PBS with 50% glycerol and 0.05% ProClin 300; pH 7.3
• Gene Accession No.: O14920, O88351, Q9QY78
• Gene Symbols: Ikbkb
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400-669 of human IKK beta (NP_0015471).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG