IKK beta Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA519916

Product Code. 12828132

Description

In Western blot applications, this antibody detects a band at ∽87kDa. A suggested positive control is Jurkat cell lysate. PA5-19916 can be used with blocking peptide PEP-0042.

Phosphorylation of serine residues on the I-kappa-B proteins by IKK kinases, including IKBKB, marks them for destruction via the ubiquitination pathway, allowing activation of the NF-kappa-B complex. Inhibitor of nuclear factor kappa-B kinase subunit beta (IKK beta) is a serine kinase that plays an essential role in the NF-kaapa-B signaling pathway which is activated by mulitple stimuli such as inflammatory cytokines, bacterial, or viral products, DNA damages, or other cellular stresses. IKK beta acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Mutations affecting the IKK beta gene can result in Immunodeficiency 15 (IMD15).

Specifications

• Antigen: IKK beta
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Ikbkb
• Gene Alias: AI132552; AIM-1; FLJ40509; I-kappa-B kinase 2; I-kappa-B-kinase beta; Ikbkb; IKK[b]; IKK2; IKK-2; IKKB; IKK-B; IKKbeta; IKK-beta; IMD15; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of kappaB kinase beta; inhibitor of nuclear factor kappa B kinase beta subunit; inhibitor of nuclear factor kappa-B kinase subunit beta; MGC131801; NFKBIKB; nuclear factor NF-kappa-B inhibitor kinase beta
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3551
• Content And Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
• Form: Liquid

• Applications: Immunocytochemistry, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: O14920
• Gene Symbols: Ikbkb
• Immunogen: A peptide corresponding to amino acids near the carboxy terminus of human IKK beta
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG