Invitrogen™ IKK beta Monoclonal Antibody (J.414.5)

Rabbit Monoclonal Antibody

Brand:  Invitrogen™ MA515080

Product Code. 11515051

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with IKK-alpha or IKK-gamma.

Phosphorylation of serine residues on the I-kappa-B proteins by IKK kinases, including IKBKB, marks them for destruction via the ubiquitination pathway, allowing activation of the NF-kappa-B complex. Inhibitor of nuclear factor kappa-B kinase subunit beta (IKK beta) is a serine kinase that plays an essential role in the NF-kaapa-B signaling pathway which is activated by mulitple stimuli such as inflammatory cytokines, bacterial, or viral products, DNA damages, or other cellular stresses. IKK beta acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Mutations affecting the IKK beta gene can result in Immunodeficiency 15 (IMD15).

Specifications

• Antigen: IKK beta
• Classification: Monoclonal
• Concentration: 56 μg/mL
• Formulation: 0.01M HEPES with 100 μg/mL BSA, 50% glycerol, 0.15M NaCl and <0.02% sodium azide; pH 7.5
• Gene Accession No.: O14920, O88351, Q9QY78
• Gene Symbols: Ikbkb
• Immunogen: Synthetic peptide corresponding to residues at the carboxy terminus of human IKKβ protein.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Monkey
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot
• Clone: J.414.5
• Conjugate: Unconjugated
• Gene: Ikbkb
• Gene Alias: AI132552; AIM-1; FLJ40509; I-kappa-B kinase 2; I-kappa-B-kinase beta; Ikbkb; IKK[b]; IKK2; IKK-2; IKKB; IKK-B; IKKbeta; IKK-beta; IMD15; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of kappaB kinase beta; inhibitor of nuclear factor kappa B kinase beta subunit; inhibitor of nuclear factor kappa-B kinase subunit beta; MGC131801; NFKBIKB; nuclear factor NF-kappa-B inhibitor kinase beta
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16150, 3551, 84351
• Content And Storage: -20°C
• Form: Liquid