Invitrogen™ IgM Monoclonal Antibody (SA-DA4), APC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Product Code	Brand	Quantity
15568296	Invitrogen™ 17-9998-41	25 Tests
15578296	Invitrogen™ 17-9998-42	100 Tests

Description

Description: The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells. Our testing has revealed variations in staining of the non-B cell population that is predicted to be non-specific. This variation can be attributed to both donor variability and the anticoagulant used during blood draw. Therefore, we recommend using sodium citrate as the anticoagulant to minimize non-specific staining by this antibody. Applications Reported: This SA-DA4 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections. Applications Tested: This SA-DA4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

Specifications

• Antigen: Human IgM
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Alias: AGM1; IGHM; immunoglobulin heavy constant mu; MU; VH
• Gene Symbols: IGHM
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: IGHM

• Clone: SA-DA4
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: P01871
• Gene ID (Entrez): 3507
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.