IgG2b Rat, Biotin, Clone: RTK4530, Isotype Control, STEMCELL Technologies

Rat Monoclonal Antibody

Product Code	Brand	Quantity
17178911	Stemcell Technologies 60077BT.1	50 μg
17168911	Stemcell Technologies 60077BT	200 μg

Description

• The RTK4530 antibody (IgG2b, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen-specific antibody
• Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being used
• If a conjugated antibody is used, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen
• The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody and indicates non-specific binding that may result from binding to Fc receptors or other cell components
• This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining)

Specifications

• Antigen: IgG2b
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence
• Conjugate: Biotin
• Isotype: IgG2b κ

• Clone: RTK4530
• Classification: Monoclonal
• Host Species: Rat
• Primary or Secondary: Isotype Control (Primary)