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Rat IgG2b kappa Isotype Control (eB149/10H5), APC-eFluor™ 780, eBioscience™, Invitrogen™
Rat Isotype Control
Brand: Invitrogen 47-4031-82
Description
Description: The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: APC-eFluor™ 780 Rat IgG2b Isotype Control (APC-Alexa Fluor™ 750 replacement) has been reported for use in flow cytometric analysis. Applications Tested: Rat IgG2b K Isotype Control APC-eFluor™ 780 has been tested by flow cytometric analysis of mouse splenocytes. Use isotype control at the same concentration as experimental antibody. APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.
Rat IgG2b is a protein-coding gene that encodes for the immunoglobulin G2b (IgG2b) subclass of rat antibodies. IgG2b is a major subclass of rat immunoglobulins that plays a crucial role in the immune response by neutralizing pathogens and promoting opsonization. Rat IgG2b is commonly used in research applications, such as in immunohistochemistry, flow cytometry, and Western blotting.Specifications
Rat IgG2b kappa | |
eB149/10H5 | |
APC-eFluor 780 | |
IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2 | |
Affinity chromatography | |
RUO | |
Not Applicable | |
Liquid |
Control, Flow Cytometry | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
Rat | |
100 μg | |
Isotype Control (Primary) | |
4° C, store in dark, DO NOT FREEZE! | |
IgG2b κ |
For Research Use Only.