Learn More
Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™, Invitrogen™
Rat Isotype Control
Brand: Invitrogen 15-4321-81
Description
Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin. Applications Reported: This rat IgG2a isotype control has been reported for use in flow cytometric analysis and ELISA. Applications Tested: Rat IgG2a Isotype Contrl is offered in 2 formats: - μg size: This can be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by staining and flow cytometric analysis. This can be used at test size: 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.
Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG2a isotype control has been reported for use in flow cytometric analysis and ELISA.Applications Tested: Rat IgG2a Isotype Contrl can be used at the same concentration as the experimental antibody.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL cell sample + 100μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.Specifications
Rat IgG2a kappa | |
eBR2a | |
PE-Cyanine5 | |
IgG2, IgG2a, IgG2k | |
Affinity chromatography | |
RUO | |
None | |
Liquid |
Flow Cytometry | |
0.2mg/mL | |
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
Rat | |
50 μg | |
Isotype Control (Primary) | |
4° C, store in dark, DO NOT FREEZE! | |
IgG2a κ |
For Research Use Only.