Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™, Invitrogen™

Rat Isotype Control

Brand:  Affymetrix eBioscience 15-4321-81

Product Code. 15300520

Description

Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin. Applications Reported: This rat IgG2a isotype control has been reported for use in flow cytometric analysis and ELISA. Applications Tested: Rat IgG2a Isotype Contrl is offered in 2 formats: - µg size: This can be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by staining and flow cytometric analysis. This can be used at test size: 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG2a isotype control has been reported for use in flow cytometric analysis and ELISA.Applications Tested: Rat IgG2a Isotype Contrl can be used at the same concentration as the experimental antibody.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL cell sample + 100μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: Rat IgG2a kappa
• Clone: eBR2a
• Conjugate: PE-Cyanine5
• Gene Alias: IgG2, IgG2a, IgG2k
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: None
• Form: Liquid

• Applications: Flow Cytometry
• Concentration: 0.2mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Host Species: Rat
• Quantity: 50 μg
• Primary or Secondary: Isotype Control (Primary)
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Isotype: IgG2a κ

For Research Use Only.