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Mouse IgG1 kappa, PE-Cyanine5, clone: P3.6.2.8.1, Isotype Control, eBioscience™

Mouse Isotype Control

Brand:  Affymetrix eBioscience 15-4714-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15567756

  • £95.25 / 100 tests


EU Stock 1
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For Research Use Only.


Description

Description

Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: PE-Cy5 Mouse IgG1 K Isotype Control has been reported for use in flow cytometric analysis.Applications Tested: Thhis Mouse IgG1 K Isotype Control is offered in 2 formats: - μg size: This can be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by staining and flow cytometric analysis. This can be used at test size: 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IgG1, K
P3.6.2.8.1
PE-Cyanine5
Conjugated
IgG1
RUO
Monoclonal
Flow Cytometry
0.2mg/mL; 5μL (0.25μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
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