Invitrogen™ Mouse IgD Monoclonal Antibody (11-26c (11-26)), NovaFluor™ Yellow 810

Rat Monoclonal Antibody

Brand:  Invitrogen™ M030T02Y09-A

Product Code. 30484927

Description

Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells. This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer. Applications Tested: This 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest Master mixes. Whole Blood compatibility: When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates.

This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.

Specifications

• Antigen: Mouse IgD
• Classification: Monoclonal
• Conjugate: NovaFluor Yellow 810
• Gene: Ighd
• Gene Alias: IgD; Igh-5; IGHD; immunoglobulin heavy constant delta
• Host Species: Rat
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: 11-26c (11-26)
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P01881
• Gene Symbols: Ighd
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 380797
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid