IgD Monoclonal Antibody (11-26c (11-26)), NovaFluor™ Yellow 610, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Invitrogen M030T02Y03

Product Code. 17870951

Description

The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells. Applications Reported: This 11-26c (11-26) antibody has been reported for use in flow cytometric analysis. Applications Tested: This 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes Our internal testing shows that NovaFluor Yellow 610 non-specifically stains B cells in SJL mice. Non-specific staining has not been observed in BALB/c or C57BL/6 mice. Other strains have not been tested. See the Antibody Testing Data for an example of this strain-dependent difference. NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes. Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer. Use 5 μL of CellBlox Blocking Buffer per stained cell sample containing 10^3 to 10^8 cells. NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Excitation: 552 nm; Emission: 612 nm; Laser: 561 nm (Yellow) Laser

This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.

Specifications

• Antigen: IgD
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P01881
• Gene Symbols: Ighd
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 380797
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 11-26c (11-26)
• Conjugate: NovaFluor Yellow 610
• Gene: Ighd
• Gene Alias: IgD; Igh-5; IGHD; immunoglobulin heavy constant delta
• Host Species: Rat
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ