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IFN gamma, clone: XMG1.2, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-7311-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15248057

  • £49.26 / 50µg


Estimated Shipment
29-03-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The XMG1.2 antibody reacts with mouse interferon (IFN) gamma. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN gamma is a 20kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN gamma, in synergy with other cytokines such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.Applications Reported: The XMG1.2 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, immunoblotting (WB), and for neutralization of IFN gamma bioactivity.Applications Tested: The XMG1.2 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IFN gamma in combination with the biotin R4-6A2 (13-7312) antibody for detection and recombinant mouse IFN gamma (39-8311) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.25-1 μg/mL. Excess amounts of XMG1.2 may significantly reduce ELISA sensitivity. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IFN gamma
XMG1.2
Unconjugated
Purified
Rat
50μg
Store at 2-8°C.
Monoclonal
ELISA Capture
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Interferon-gamma, IFN-g, IFNg
IgG1
RUO
Primary
Murine
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