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IFN gamma, clone: NIB42, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-7318-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15208067

  • £49.94 / 50µg


EU Stock 1
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Description

Description

Description: The NIB42 antibody reacts with human interferon-gamma (IFN-gamma). The NIB42 antibody is a neutralizing antibody. IFN-gamma is an ∽20kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-gamma in synergy with other cytokines, such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity.Applications Reported: The NIB42 antibody has been reported for use in capture of human IFN gamma by ELISA, and for neutralizing IFN gamma bioactivity. Use Functional Grade purified for functional assays cat. 16-7318).Applications Tested: The NIB42 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interferon-gamma (IFN-g) in combination with the biotin 4S.B3 (13-7319) antibody for detection and recombinant human IFNg (29-8319) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 2-8 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.For ELISPOT Capture, the alternative clone MD-1 is recommended.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

IFN gamma
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P01579
Interferon gamma
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
ELISA
NIB42
Unconjugated
IFNG
Purified
IFNG
IgG1, kappa
50μg
Store at 2-8°C.
Primary
3458
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