Invitrogen™ ICAD Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA534694

Product Code. 15388561

Description

Recommended positive controls: 293T, A431, Jurkat, Raji. Predicted reactivity: Mouse (81%), Rat (83%), Rhesus Monkey (94%), Bovine (80%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

ICAD is the inhibitor of caspase-3-activated DNase which is a substrate that controls nuclear apoptosis. ICAD has two isoforms: a functional isoform of 45kDa, ICAD-L/DNA fragmentation factor (DFF) 45; and a 35kDa isoform, ICAD-S/DFF35. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD. In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone. There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells. Two alternatively spliced transcript variants encoding distinct isoforms have been found for the ICAD gene.

Specifications

• Antigen: ICAD
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DFFA
• Gene Alias: A330085O09Rik; DFF; DFF1; DFF35; Dff45; DFF-45; Dffa; DNA fragmentation factor 45 kDa subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor, 45kDa, alpha polypeptide; DNA fragmentation factor, alpha subunit; H13; ICAD; ICAD-L; ICAD-S; Inhibitor of CAD; RP23-121D17.3
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 114214, 13347, 1676
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: O00273, O54786
• Gene Symbols: DFFA
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ICAD. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG