Invitrogen™ ICAD (cleaved Asp224) Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA577941

Product Code. 16316654

Description

Positive Control: Jurkat, Jurkat (10uM etoposide treatment for 24h) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Specifications

• Antigen: ICAD (cleaved Asp224)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: DFFA
• Gene Alias: DFF1; DFF45; DFF-45; DFFA; DNA fragmentation factor 45 kDa subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor, 45kDa, alpha polypeptide; H13; ICAD; Inhibitor of CAD
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1676
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.73 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: O00273
• Gene Symbols: DFFA
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ICAD (cleaved Asp224). The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG