Nitrotryptophan Monoclonal Antibody (4F8), APC, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA545821

Product Code. 17836682

Description

A 1:1,000 dilution of MA5-45821 was sufficient for detection of 6-Nitrotryptophan in 1 μg of 6-Nitrotyrptophan conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.∣Specific for 6-Nitrotyrptophan modified proteins. Doss not cross-react with 7-Ketocholoesterol modified proteins.

6-nitrotryptophan (6-NO2-Trp) is shown to be one of the most stable and major ONOO- modified amino acids, and growing evidences suggest that nitrotryptophan may play a significant role in the cellular regulation under nitrosative stress. Reactive nitrogen species (RNS) are formed in a variety of diseases such as atherosclerosis, inflammation, cancer and neurodegenerative disiases. Peroxinitrite (ONOO-) is known as a potent biological oxidant, which is formed via very fast reaction between nitric oxide (NO) and superoxide (O2-). Peroxinitrite can induce nitration of aromatic amino acids such as Tyr, Trp, Phe and His, which results in modulation of protein functions.

Specifications

• Antigen: Nitrotryptophan
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.15mM MES, 47.35mM phosphate with 1.033mM EDTA and no preservative; pH 7.4
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Chemical
• Form: Liquid
• Target Molecule: Chemical

• Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Western Blot
• Clone: 4F8
• Conjugate: APC
• Gene Alias: 6-Nitrotryptophan; 6-NO2-Trp; Nitrotryptophan; NW
• Immunogen: Synthetic 6-Nitrotryptophan modified Keyhole Limpet Hemocyanin (KLH).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: 4° C
• Isotype: IgG2b